calfluor 647 azide (Click Chemistry Tools)

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Click Chemistry Tools calfluor 647 azide
Calfluor 647 Azide, supplied by Click Chemistry Tools, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/calfluor+647+azide/calfluor+647+azide/pm40410175-302-34-37
Average 90 stars, based on 1 article reviews

calfluor 647 azide - by Bioz Stars, 2026-09

90/100 stars

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Labeling:

Article Title: Inhibiting O-GlcNAcylation impacts p38 and Erk1/2 signaling and perturbs cardiomyocyte hypertrophy.
Article Snippet: .. For the labeling of glycosylated proteins with CalFluor 647 azide, the copper-catalyzed alkyne-azide reaction contained 20 μg protein suspension in 0.6% SDS, 2 mM sodium ascorbate, 100 μM THPTA, 1 mM CuSO4.5H2O, and either 20 μM or 50 μM CalFluor 647 azide (Cat. No. 1372, Click Chemistry Tools). ..

Article Title: Inhibiting O-GlcNAcylation impacts p38 and Erk1/2 signaling and perturbs cardiomyocyte hypertrophy
Article Snippet: .. For the labeling of glycosylated proteins with CalFluor 647 azide, the copper-catalyzed alkyne-azide reaction contained 20 μg protein suspension in 0.6% SDS, 2 mM sodium ascorbate, 100 μM THPTA, 1 mM CuSO 4 .5H 2 O, and either 20 μM or 50 μM CalFluor 647 azide (Cat. No. 1372, Click Chemistry Tools). ..

Suspension:

Incubation:

Article Title: inCu-click: DNA-enhanced ligand enables live-cell, intracellular click chemistry reaction with copper catalyst.
Article Snippet: About 2.5μl of Ac4ManNAl (5mM; Click Chemistry Tools) was added to each well of the 18-well chamber (Cellvis) and dried for 20min. .. Cells were seeded into the Ac4ManNAltreated 18-well chamber, and the total volume per well was 150μl, and incubated for 24 h. Cells were washed 3X with PBS/1%FBS (pH 7.4) followed by treatment with 10μM CalFluor 647 Azide (Click Chemistry Tools), premixed BTTAA-CuSO4 complex ([BTTAA]:[CuSO4] = 2:1, [CuSO4] = 10μM)or BTT-DNA ligand ([BTT-DNA] = 20μM) and 2.5mM freshly prepared sodium ascorbate for 30min on ice. .. The cells were washed 3Xwith 200μl of PBS/1%FBS (pH 7.4) to remove the unreacted CalFluor 647 Azide dye and followed by staining with 100μl of diluted ReadyProbesTM Cell Viability Imaging Kit, Blue/Green (Thermo Fisher Scientific) for 10min at 37 °C to stain the nucleus of the live and dead cells.

Article Title: inCu-click: DNA-enhanced ligand enables live-cell, intracellular click chemistry reaction with copper catalyst
Article Snippet: About 2.5 μl of Ac 4 ManNAl (5 mM; Click Chemistry Tools) was added to each well of the 18-well chamber (Cellvis) and dried for 20 min. .. Cells were seeded into the Ac 4 ManNAl-treated 18-well chamber, and the total volume per well was 150 μl, and incubated for 24 h. Cells were washed 3X with PBS/1%FBS (pH 7.4) followed by treatment with 10 μM CalFluor 647 Azide (Click Chemistry Tools), premixed BTTAA-CuSO 4 complex ([BTTAA]:[CuSO 4 ] = 2:1, [CuSO 4 ] = 10 μM) or BTT-DNA ligand ([BTT-DNA] = 20 μM) and 2.5 mM freshly prepared sodium ascorbate for 30 min on ice. .. The cells were washed 3X with 200 μl of PBS/1%FBS (pH 7.4) to remove the unreacted CalFluor 647 Azide dye and followed by staining with 100 μl of diluted ReadyProbesTM Cell Viability Imaging Kit, Blue/Green (Thermo Fisher Scientific) for 10 min at 37 °C to stain the nucleus of the live and dead cells.

other:

Article Title: inCu-click: DNA-enhanced ligand enables live-cell, intracellular click chemistry reaction with copper catalyst.
Article Snippet: Cellswere seeded into an 18-well chamber (Cellvis), and 1.5μl of propargyl choline (10mM; Jena Bioscience) was added to each well, and the total volume per well was 150μl, and incubated for 24 h. Cells were washed 3X with PBS/1%FBS (pH 7.4) followed by treatment with 10μMCalFluor 647 Azide (Click Chemistry Tools), premixed BTTAA-CuSO4 complex ([BTTAA]:[CuSO4] = 2:1, [CuSO4] = 10μM) or BTT-DNA ligand ([BTT-DNA] = 20μM) and 2.5mM freshly prepared sodium ascorbate for 30min on ice.

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